Christian Poüs

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: It is demonstrated that Golgi-based microtubule nucleation is direct and involves a subset of gamma-tubulin bound to the cytoplasmic face of the organelle.

TL;DR: Observations in living cells suggested that GTP remnants may be responsible for the rescue events in which microtubules recover from catastrophe, and a recombinant antibody was selected that specifically recognizes GTP-bound tubulin in micro Tubules and found that G TP-tubulin was indeed present at the plus end of growing microtubule.

TL;DR: This review highlights the emerging roles of tubulin acetylation in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signalling and points out that tubulinacetylation should no longer be seen as a passive marker of microtubule stability, but as a broad regulator of micro tubule functions.

TL;DR: Spatial and temporal coordination of many stages in the life of autophagosomes underlines the integrative role of microtubules and progressively reveals hidden parts of the autophagy machinery.