Daniel J. Ledbetter

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The two prominent recombination systems are Cre-lox, isolated from bacteriophage, and FLP-FRT, originating in yeast as mentioned in this paper, which have been utilized in a vast array of cell lines including embryonic stem (ES) cells, and hundreds of transgenic mouse lines for tailored gene modification strategies.

TL;DR: This chapter is structured with the goal of providing a background of these and other recombination systems, information on protein enhancements designed to modify the functionality or utility of these systems, and a protocol-driven set of methods to perform and detect site-specific recombination in ES cells.

TL;DR: Tatsui et al. as discussed by the authors investigated the anti-mitotic effect of TTF to inhibit the growth of human cancer cells in vitro and in vivo models of spinal metastasis.