Nelson Ruiz-Opazo

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The culture conditions found to result in stable proliferation of normal rat hepatocytes are: subconfluent cell densities; serum-free medium; hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and substrata of liver extracellular matrix depleted of growth inhibitors.

TL;DR: The cloned cDNAs as probes have isolated the single gene locus encoding both MLC1f and MLC3f in four overlapping genomic clones spanning over approximately 25 kilobase pairs (kb) of DNA, implying a novel form of alternative promoter utilization and RNA splicing that is tissue-specific and developmentally regulated in order to generate the mature MLC first and M LC3f mRNAs from a single gene.

TL;DR: Evidence is presented in PLC/PRF/5 cells for integration of HBV DNA into the host genome and expression of three RNA molecules containing HBV-specific sequences, consistent with observations in several animal virus models in which integrated viral DNA is found during oncogenic transformation.

TL;DR: Structural analysis of the alpha subunits from rat and other species predicts a polytopic protein with seven membrane-spanning regions, which may provide a biochemical basis for Na,K-ATPase functional diversity.