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Mitsuhiro Yanagida

Researcher at Okinawa Institute of Science and Technology

Publications -  309
Citations -  33295

Mitsuhiro Yanagida is an academic researcher from Okinawa Institute of Science and Technology. The author has contributed to research in topics: Schizosaccharomyces pombe & Mitosis. The author has an hindex of 94, co-authored 306 publications receiving 31224 citations. Previous affiliations of Mitsuhiro Yanagida include University of Manchester & Nara Institute of Science and Technology.

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Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy

Daniel J. Klionsky, +1287 more
- 01 Apr 2012 - 
TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
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CRM1 is responsible for intracellular transport mediated by the nuclear export signal

TL;DR: It is shown that p110 is CRM1, which is an evolutionarily conserved protein originally found as an essential nuclear protein in fission yeast and known as a likely target of LMB, which indicates that CRM 1 is an essential mediator of the NES-dependent nuclear export of proteins in eukaryotic cells.
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Leptomycin B Inhibition of Signal-Mediated Nuclear Export by Direct Binding to CRM1

TL;DR: Evidence is shown that LMB binds directly to CRM1 and thatCRM1 is essential for NES-dependent nuclear export of proteins in both yeast and mammalian cells, and is an essential factor for nuclear exported proteins in eukaryotes.
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DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe.

TL;DR: It is shown that DNA topoisomerase II (topo II) is continuously required for mitotic chromosome changes in Schizosaccharomyces pombe and pulse-shift experiments show that topo II is required for chromatid disjuction.
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Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

TL;DR: Fluorescence in situ hybridization (FISH) shows that fission yeast centromeres and telomeres make up specific spatial arrangements in the nucleus and is a powerful tool for analyzing mitotic chromosome movement and disjunction using various mutants.