Virginia Espina

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: A thorough description of laser-capture microdissection techniques is provided, with an emphasis on tips and troubleshooting advice derived from LCM users.

TL;DR: A protocol for making reverse-phase protein lysate microarrays with larger numbers of spots than previously feasible is developed, and a striking regularity appeared: cell-structure-related proteins almost invariably showed a high correlation between mRNA and protein levels across the NCI-60 cell lines, whereas non-cell-st structure- related proteins showed poor correlation.

TL;DR: Protein microarrays, one emerging class of proteomic technologies, have broad applications for discovery and quantitative analysis and is now employed for the analysis of biopsy samples in clinical trial research.

TL;DR: Adipocytes play a vital role in defining the ECM environment for normal and tumor-derived ductal epithelial cells and contribute significantly to tumor growth at early stages through secretion and processing of collagen VI.