Kohki Yoshimoto

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The phenotypic analysis of atg44a4b-1 indicated that plant autophagy contributes to the development of a root system under conditions of nutrient limitation and biochemical analyses provided evidence that autophagic bodies in the vacuoles of wild-type roots are detected but not in those of atG4a-1 in the presence of concanamycin A, a V-ATPase inhibitor.

TL;DR: A conserved requirement for salicylic acid (SA) signaling for senescence and immunity-related programmed cell death phenomena in autophagy-defective mutants (atg mutants) is demonstrated and it is shown that autophagic is induced by the SA agonist.

TL;DR: It is concluded that Rubisco and stroma-targeted fluorescent proteins can be mobilized to the vacuole through an ATG gene-dependent autophagic process without prior chloroplast destruction.

TL;DR: In this article, the uptake and degradation of cytoplasmic material by vacuolar autophagy in plants has been studied extensively by electron microscopy and shown to be involved in developmental processes such as vacuole formation, deposition of seed storage proteins and senescence, and in response of plants to nutrient starvation and to pathogens.