Marcus Fechheimer

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: Apoptosis, a morphologically and biochemically defined type of programmed cell death commonly seen in vertebrates, was found to be initiated during baculovirus replication in insect cells and a specific viral gene product, p35, was identified as being responsible for blocking the apoptotic response.

TL;DR: Scrap loading and sonication loading are two recently described methods of introducing macromolecules into living cells as discussed by the authors, which can be employed to generate cell lines that express the gene product of the transfected DNA molecules both transiently and stably.

TL;DR: The data indicate that although CDPK does not interact directly with actin, it may be associated with an actin-binding protein and therefore could play a role in the regulation of the plant cytoskeleton.

TL;DR: The use of a fluorescent derivative of vitamin D-binding protein as a specific probe for unpolymerized actin in cultured vertebrate cells is reported, enabling direct comparisons of the distribution of unpolymersed actin to that of microinjected fluorescent actin (total actin) and to actin filaments stained with phalloidin.