Luca Benetti

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: It is reported that the autophagic degradation pathway was induced at late time points after VZV infection of cultured cells, as documented by immunoblot analysis of the cellular proteins LC3B and p62/SQSTM1, along with electron microscopy analysis, which demonstrated the presence of both early autophagosomes and late autophile compartments.

TL;DR: It is suggested that autophagy was a common event in VZV-infected cells and that it was provoked at least in part by ER stress secondary to overly abundant V zoster glycoprotein biosynthesis, which led to UPR activation in an attempt to maintain cellular homeostasis.

TL;DR: The need for standard reference reagents, well-annotated databases, large data storage and transfer capacity, personnel with relevant expertise, and harmonization of international regulations for testing biologic products and reagents used for their manufacturing was identified.

TL;DR: Common strategic approaches for setting specifications for standard biotherapeutic products such as monoclonal antibodies are captured and includes considerations for ensuring specifications are patient centric.