Tomohiro Yorimitsu

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The results indicate that purified Sec16 alone can self-assemble into homo-oligomeric complexes on a planar lipid membrane and plays an important role in regulating Sar1 GTPase activity at the late steps of COPII vesicle formation.

TL;DR: This review focuses on the current knowledge of Sar/Arf GTPases, highlighting the molecular details of GTPase regulation in vesicle formation in yeast and advances in knowledge of the characteristics of vesicles trafficking in plants.

TL;DR: The loss of REI-1 specifically impaired the targeting of RAB-11 to the late-Golgi compartment and the recycling endosomes in embryos and further reduced the RAB -11 distribution to the cleavage furrow, which resulted in cytokinesis delay, suggesting that REi-1 is a GEF specifically regulating the R AB-11 localization and functions in early embryos.

TL;DR: Findings point to an as-yet-undefined role of COPII proteins in the formation of ERACs, which are segregated into specialized ER subdomains, termed ER-associated compartments (ERACs) before degradation.