Ana Serrano-Puebla

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The role of autophagy in retinal development and cell differentiation is described, and the implications of Autophagy dysregulation both in physiological aging and in important diseases such as age-associated macular degeneration and glaucoma are discussed.

TL;DR: The latest evidence on LMP‐mediated cell death, the upstream and downstream signals involved, and the role of LMP in the normal physiology of organisms is reviewed.

TL;DR: It is shown that THC (but not nutrient deprivation) increases the dihydroceramide:ceramide ratio in the endoplasmic reticulum of glioma cells, and this alteration is directed to autophagosomes and autolysosomes to promote lysosomal membrane permeabilization, cathepsin release and the subsequent activation of apoptotic cell death.

TL;DR: This work describes a flow cytometry-based approach to determine mitophagy by using MitoTracker Deep Red, a widely used mitochondria-selective probe used in combination with selective inhibitors, and test the validity of the use of this method in cell lines and in primary cell and tissue cultures.