Karol Dokladny

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The data indicate that the IL-1β increase in Caco-2 TJ permeability was mediated by an increase in MLCK expression and activity, and that this mediated an NF-κB-dependent increase inMLCK gene transcription.

TL;DR: It is demonstrated for the first time that a modest, physiologically relevant increase in temperature causes an increase in intestinal epithelial TJ permeability and suggested that HSP mediated upregulation of occludin expression may be an important mechanism involved in the maintenance of intestinal epithelium TJ barrier function during heat stress.

TL;DR: The effects of exertional or nonexertional (passive hyperthermia) heat stress on tight junction barrier function in in vitro and in vivo models and changes in tight junction proteins in response to exercise or hyperthermic conditions are reviewed.

TL;DR: Studying the links between exercise stress and molecular control of proteostasis provides evidence that the heat shock response and autophagy coordinate and undergo sequential activation and downregulation, and that this is essential for proper proteostotic systems in eukaryotic systems.