Marcos P. Thomé

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: The photochemical basis of Acridine Orange (AO) is described and its usefulness as a probe for autophagy quantification is found and it is found that considering the red-to-green fluorescence intensity ratio of AO gives the best correlation with other autophagic assays.

TL;DR: The presence of Rsv forces cells treated with TMZ through mitosis leading to mitotic catastrophe and senescence, reducing the clonogenic capacity of glioma cells and increasing the chronic effects of temozolomide.

TL;DR: It is demonstrated that acute treatment with temozolomide induces DNA damage, a transitory activation of PRKAA/AMPK-ULK1 and MAPK14/p38 and the sustained inhibition of AKT-MTOR produces a transient induction of autophagy, which is followed by senescence, showing that DNA damage-induced Autophagy acts by suppressing apoptosis.