Roberta Nardacci

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: It is shown that Ambra1 (activating molecule in Beclin1-regulated autophagy), a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulatesAutophagy and has a crucial role in embryogenesis, and provides in vivo evidence supporting the existence of a complex interplay between autphagy, cell growth and cell death required for neural development in mammals.

TL;DR: When autophagy is induced, ULK1 phosphorylates AMBRA1, releasing the autophagic core complex from the cytoskeleton and allowing its relocalization to the ER membrane to nucleate autophagosome formation.

TL;DR: It is shown that, upon mitophagy induction, AMBRA1 binds the autophagosome adapter LC3 through a LIR (LC3 interacting region) motif, this interaction being crucial for regulating both canonical PARKIN-dependent and -independent mitochondrial clearance.