Patricia Silvia Romano

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: It is proposed that C.’burnetii transits through the normal endo/phagocytic pathway but actively interacts with autophagosomes at early times after infection, possibly favouring the intracellular differentiation and survival of the bacteria.

TL;DR: Experimental and genomic data indicating that the canonical autophagy machinery characterized in animals and fungi appeared prior to the radiation of major eukaryotic lineages are discussed, and comparative bioinformatics revealed that this canonical machinery has been subject to moderation, outright loss or elaboration on multiple occasions in protist lineages.

TL;DR: It is shown, for the first time, that the vacuole containing T. cruzi (TcPV) is decorated by the host cell autophagic protein LC3, and live cell imaging experiments indicate that autolysosomes are recruited to parasite entry sites.