Shuyan Lu

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: The assay is a robust, rapid screen that can be used to identify lysosomotropic, as well as, cytotoxic compounds, and can be positioned within a screening paradigm to understand the role of lysOSomotropism as a contributor to drug-induced toxicity.

TL;DR: This work highlights a pathway of response to lysosomal stress and evidences the role of TFEB, TFE3 and MITF in the stress response and implicates calcium and mTORC1 modulation involvement in the adaptive changes.