Anuj Kumar

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: This study reports the first proteome-scale analysis of protein localization within any eukaryote, and presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast.

TL;DR: This work has developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and has applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae.

TL;DR: The problems in defining the extent of the proteomes for completely sequenced eukaryotic organisms, focusing on yeast, worm, fly and human, are discussed, and the current estimates for the numbers of human genes are surveyed and a range for the size of the human proteome is estimated.

TL;DR: Arabidopsis genes that encode the enzymes of the C5 pathway via functional complementation of mutations in the corresponding genes of E. coli were isolated and RNA gel blot analyses indicated that transcripts for both genes are found in root, leaf, stem, and flower tissues and that their levels are dramatically elevated by light.