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Jose M. Seguí-Simarro

Researcher at Polytechnic University of Valencia

Publications -  88
Citations -  8048

Jose M. Seguí-Simarro is an academic researcher from Polytechnic University of Valencia. The author has contributed to research in topics: Microspore & Doubled haploidy. The author has an hindex of 29, co-authored 84 publications receiving 7278 citations. Previous affiliations of Jose M. Seguí-Simarro include Spanish National Research Council & University of Colorado Boulder.

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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky, +2522 more
- 21 Jan 2016 - 
TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
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Electron Tomographic Analysis of Somatic Cell Plate Formation in Meristematic Cells of Arabidopsis Preserved by High-Pressure Freezing

TL;DR: This work has investigated the process of somatic-type cytokinesis in Arabidopsis meristem cells with a three-dimensional resolution of ∼7 nm by electron tomography of high-pressure frozen/freeze-substituted samples, and demonstrated that this process can be divided into four phases: phragmoplast initials, solid phrgmoplast, transitional phragoplast, and ring-shaped phragioplast.
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Human papillomavirus L1 protein expressed in tobacco chloroplasts self-assembles into virus-like particles that are highly immunogenic.

TL;DR: The results predict a promising future for the development of a plant-based vaccine against HPV, as leaf protein extracts from L1 transgenic plants were highly immunogenic in mice after intraperitoneal injection, and neutralizing antibodies were detected.
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How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis.

TL;DR: This review compile the most recent advances in the understanding of the changes undergone by the induced microspore to readapt to the new developmental scenario, with special attention to the efforts made to uncover changes in the transcriptome of the inducedmicrospore and microSpore-derived embryo (MDE).
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Cell cycle-dependent changes in Golgi stacks, vacuoles, clathrin-coated vesicles and multivesicular bodies in meristematic cells of Arabidopsis thaliana : A quantitative and spatial analysis

TL;DR: The vacuole system undergoes major changes in organization, size, and volume, with the most notable change seen during early telophase cytokinesis, where the vacuoles form sausage-like tubular compartments with a 50% reduced surface area and an 80% reduced volume.