Bradford G. Hill

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: Methods for assessing the impact of reactive species on isolated mitochondria are described using representative cell types from renal, cardiovascular, nervous, and tumorigenic model systems while illustrating the application of three protocols to analyze the bioenergetic response of cells to oxidative stress.

TL;DR: The integration and interpretation of cellular bioenergetics in the context of mitochondrial quality control and genetics is the theme of this review.

TL;DR: Exposure to nontoxic concentrations of NO or low levels of hydrogen peroxide generated from 2,3-dimethoxy-1,4-napthoquinone (DMNQ) had little impact on basal mitochondrial function but both treatments reversibly decreased mitochondrial reserve capacity; combined NO and DMNQ treatment resulted in an irreversible loss of reserve capacity and was associated with cell death.

TL;DR: This minireview emphasizes the current understanding of the biochemistry of NO and place it in a biological context and highlights a growing importance of the secondary metabolites of NO-dependent reactions in the post-translational modification of key metabolic and signaling proteins.