Yongchao Zhao

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: It is reported that DEPTOR is a physiological substrate of SCF(βTrCP) E3 ligase for targeted degradation by SCF E3 for ubiquitin-proteasome pathway degradation.

TL;DR: The UPS and CRL E3s are introduced and the biological processes regulated by each of eight CRLs through substrate degradation are discussed, including how cullin neddylation controls CRL activity, and how CRL's are being validated as the attractive cancer targets by abrogating the RING component through genetic means and by inhibiting cullinNEDylation via MLN4924, a small molecule indirect inhibitor of CRLS, currently in several Phase I clinical trials.

TL;DR: MLN4924, a small molecule inhibitor of NAE, was discovered that inactivates CRLs and causes accumulation of CRL substrates to suppress tumor cell growth both in vitro and in vivo.