Xin Wen

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: Current knowledge about macroautophagy mainly in yeast is summarized, including the mechanism of autophagosome biogenesis, the function of the core Macroautophagic machinery, the regulation of macroAutophagy and the process of cargo recognition in selective macroautogagy are summarized, with the goal of providing insights into some of the key unanswered questions in this field.