Thomas Wollert

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: It is shown that ESCRT-0 forms domains of clustered cargo but does not deform membranes, which explains how the ESCRTs direct membrane budding and scission from the cytoplasmic side of the bud without being consumed in the reaction.

TL;DR: Three subunits of ESCRT-III, Vps20, Snf7 and Vps24, are sufficient to detach intralumenal vesicles and are reconstituted and visualized by fluorescence microscopy.

TL;DR: Analyzing the recognition complex of the listerial invasion protein InlA and its human receptor E-cadherin, two single substitutions are postulated and verified to increase binding affinity by four orders of magnitude and extend binding specificity to include formerly incompatible murine E- cadher in a versatile murine model of human listeriosis.